Sample ID: VE24-1079_COI
[analysis description + link to github repo]
| Facility | Hogwarts |
| Analyst | John Doe |
| Analysis started | 2025-05-03 01:28:09 |
| Analysis completed | 2025-05-03 01:28:09 |
| Wall time | 0:0:0 hours |
Inconclusive
Outcome: The analyst should attempt subjective species identification at the genus level.
Reasoning: [Flag 1B] 2-3 candidate species matched with high stringency (identity ≥ 98.5%).
| Preliminary morphology ID confirmed? | NA |
|
Inconclusive taxonomic identity (Flag 1B) Miridae. |
| Taxa of interest detected? | False |
|
Flag 2B: Taxon of interest NOT detected in candidate species Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries) Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus |
|
| Locus | COI |
| Preliminary ID | Miridae |
| Taxa of interest |
Lygus pratensis |
| Country | Netherlands |
| Host | Cut flower Paenonia |
| Sample ID | VE24-1079_COI |
| Query DNA sequence |
Flag 1B:
The analyst should attempt subjective species identification at the genus level
2-3 candidate species matched with high stringency (identity ≥ 98.5%)
Candidate hits must meet ONE of these criteria:
| Minimum alignment length |
400bp
|
| Minimum query coverage |
85.0%
|
Candidate hits have then been classified as follows:
| Classification | Alignment identity | Number of hits | Number of species |
|---|---|---|---|
| STRONG MATCH | ≥ 98.5% | 22 | 2 |
| MODERATE MATCH | ≥ 93.5% | NA | NA |
| NO MATCH | < 93.5% |
| Species | Hits | Identity | E-value | Database coverage |
|---|---|---|---|---|
| Orius laevigatus | 21 | 99.1% | 0.0 | |
| Orius horvathi | 1 | 98.8% | 0.0 |
This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.
| Taxon of interest | Match rank | Match taxon | Match species | Match accession | Match identity | Database coverage |
|---|---|---|---|---|---|---|
| Lygus pratensis | Did not match any candidate | |||||
This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.
Independent sources
Flag 4A:
Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources
(found 7 sources)
7 Independent Sources
The matching reference sequences for this species have been annotated by 7 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG007857 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007857 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007859 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007859 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007858 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007858 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007856 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007856 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| Show 6 more publications... | ||||
Source 2
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| PQ764849 | False |
Jeong,D. Sun,H. Kim,J. Seo,M. |
Direct Submission | Submitted (16-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea |
| PQ764848 | False |
Jeong,D. Sun,H. Kim,J. Seo,M. |
Direct Submission | Submitted (15-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea |
| PQ764850 | False |
Jeong,D. Sun,H. Kim,J. Seo,M. |
Direct Submission | Submitted (16-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea |
| Show 1 more publication... | ||||
Source 3
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| FM210187 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210187 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| FM210184 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210184 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| FM210183 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210183 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| FM210186 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210186 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| FM210185 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210185 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| Show 8 more publications... | ||||
Source 4
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| KJ467500 | False |
Pinol,J. Mir,G. Gomez-Polo,P. Agusti,N. |
Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods | Mol Ecol Resour 15 (4), 819-830 (2015) |
| KJ467500 | False |
Pinol,J. Mir,G. Gomez-Polo,P. Agusti,N. |
Direct Submission | Submitted (18-FEB-2014) Entomology, IRTA (Institute for Food and Agricultural Research and Technology), Carretera de Cabrils, Km. 2, Cabrils, Barcelona 08348, Spain |
Source 5
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| GQ292148 | False |
Jung,S. Duwal,R.K. Lee,S. |
COI barcoding of true bugs (Insecta, Heteroptera) | Mol Ecol Resour 11 (2), 266-270 (2011) |
| GQ292148 | False | Jung,S.H. | Direct Submission | Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea |
| GQ292147 | False |
Jung,S. Duwal,R.K. Lee,S. |
COI barcoding of true bugs (Insecta, Heteroptera) | Mol Ecol Resour 11 (2), 266-270 (2011) |
| GQ292147 | False | Jung,S.H. | Direct Submission | Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea |
| GQ292149 | False |
Jung,S. Duwal,R.K. Lee,S. |
COI barcoding of true bugs (Insecta, Heteroptera) | Mol Ecol Resour 11 (2), 266-270 (2011) |
| GQ292149 | False | Jung,S.H. | Direct Submission | Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea |
| Show 4 more publications... | ||||
Source 6
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MW357548 | False |
Tabic,A. Ben-Ami,R. |
Direct Submission | Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel |
| MW357547 | False |
Tabic,A. Ben-Ami,R. |
Direct Submission | Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel |
| MW357550 | False |
Tabic,A. Ben-Ami,R. |
Direct Submission | Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel |
| MW357549 | False |
Tabic,A. Ben-Ami,R. |
Direct Submission | Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel |
| Show 2 more publications... | ||||
Source 7
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG452375 | False |
Erfanfar,D. Srafrazi,A. Nouri- Ganbalani,G. Ostovan,H. Shojaei,M. |
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran | Unpublished |
| MG452375 | False |
Erfanfar,D. Sarafrazi,A. |
Direct Submission | Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran |
Independent sources
Flag 4B:
Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources
(found 1 sources)
1 Independent Source
The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG452387 | False |
Erfanfar,D. Srafrazi,A. Nouri- Ganbalani,G. Ostovan,H. Shojaei,M. |
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran | Unpublished |
| MG452387 | False |
Erfanfar,D. Sarafrazi,A. |
Direct Submission | Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran |
This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.
The following resources can be used to ensure that the given taxonomy is legitimate and current.
| Taxa | Database |
|---|---|
| General | GBIF |
| General | ITIS |
| Mealybugs & scale | ScaleNet database |
| Thrips | Thripswiki |
| Spider Mites | Spider Mites Database |
| Psocodea (Barklice, Booklice, and Parasitic Lice) | Psocodea Species File Online |
| Orthoptera | Orthoptera Species File Online |
| Drosophilidae | TaxoDros |
| Diptera |
Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum |
| Aphids | Aphid Species File |
| Ants |
AntWeb
AntCat |
| Lepidoptera (butterflies and moths) | The Global Lepidoptera Names Index |
| Gracillariidae (primitive moths) | Global Taxonomic Database of Gracillariidae |
| Pyralidae (pyralid moths) | Global Information System on Pyraloidea |
| Tortricidae (tortrix moths) | Tortricidae Resources on the Net |