Taxonomic identification report

Sample ID: VE24-1079_COI

Department of Agriculture, Fisheries and Forestry logo

[analysis description + link to github repo]

Facility Hogwarts
Analyst John Doe
Analysis started 2025-05-03 01:28:09
Analysis completed 2025-05-03 01:28:09
Wall time 0:0:0 hours

Result overview

Inconclusive

Outcome: The analyst should attempt subjective species identification at the genus level.

Reasoning: [Flag 1B] 2-3 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed? NA

Inconclusive taxonomic identity (Flag 1B) Miridae.

Taxa of interest detected? False

Flag 2B: Taxon of interest NOT detected in candidate species

Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries)

Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus

Sample details

Locus COI
Preliminary ID Miridae
Taxa of interest Lygus pratensis
Country Netherlands
Host Cut flower Paenonia
Sample ID VE24-1079_COI
Query DNA sequence

Analyst evaluation

Identification of candidate species

Flag 1B: The analyst should attempt subjective species identification at the genus level
2-3 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length 400bp
Minimum query coverage 85.0%

Candidate hits have then been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 22 2
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Orius laevigatus 21 99.1% 0.0
Orius horvathi 1 98.8% 0.0

Analyst evaluation

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Lygus pratensis Did not match any candidate

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent sources

Flag 4A: Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources (found 7 sources)

7 Independent Sources

The matching reference sequences for this species have been annotated by 7 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
MG007857 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) Bull Soc Entomol France 123 (1), 29-42 (2018)
MG007857 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Direct Submission Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France
MG007859 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) Bull Soc Entomol France 123 (1), 29-42 (2018)
MG007859 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Direct Submission Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France
MG007858 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) Bull Soc Entomol France 123 (1), 29-42 (2018)
MG007858 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Direct Submission Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France
MG007856 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) Bull Soc Entomol France 123 (1), 29-42 (2018)
MG007856 False Streito,J.-C.
Fontaine,O.
Atiama,M.
Genson,G.
Pierre,E.
Frago,E.
Nibouche,S.
Direct Submission Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France
Show Hide 6 more publications...

Source 2

Hit accession Automated Authors Title Journal
PQ764849 False Jeong,D.
Sun,H.
Kim,J.
Seo,M.
Direct Submission Submitted (16-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea
PQ764848 False Jeong,D.
Sun,H.
Kim,J.
Seo,M.
Direct Submission Submitted (15-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea
PQ764850 False Jeong,D.
Sun,H.
Kim,J.
Seo,M.
Direct Submission Submitted (16-DEC-2024) Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, 166, Nongsaengmyeong-ro, Wanju-gun, Jeollabuk-do 55365, South Korea
Show Hide 1 more publication...

Source 3

Hit accession Automated Authors Title Journal
FM210187 False Pasquer,F.
Pfunder,M.
Frey,B.
Frey,J.E.
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures Biocontrol Sci Technol (2010) In press
FM210187 False Pasquer,F. Direct Submission Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND
FM210184 False Pasquer,F.
Pfunder,M.
Frey,B.
Frey,J.E.
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures Biocontrol Sci Technol (2010) In press
FM210184 False Pasquer,F. Direct Submission Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND
FM210183 False Pasquer,F.
Pfunder,M.
Frey,B.
Frey,J.E.
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures Biocontrol Sci Technol (2010) In press
FM210183 False Pasquer,F. Direct Submission Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND
FM210186 False Pasquer,F.
Pfunder,M.
Frey,B.
Frey,J.E.
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures Biocontrol Sci Technol (2010) In press
FM210186 False Pasquer,F. Direct Submission Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND
FM210185 False Pasquer,F.
Pfunder,M.
Frey,B.
Frey,J.E.
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures Biocontrol Sci Technol (2010) In press
FM210185 False Pasquer,F. Direct Submission Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND
Show Hide 8 more publications...

Source 4

Hit accession Automated Authors Title Journal
KJ467500 False Pinol,J.
Mir,G.
Gomez-Polo,P.
Agusti,N.
Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods Mol Ecol Resour 15 (4), 819-830 (2015)
KJ467500 False Pinol,J.
Mir,G.
Gomez-Polo,P.
Agusti,N.
Direct Submission Submitted (18-FEB-2014) Entomology, IRTA (Institute for Food and Agricultural Research and Technology), Carretera de Cabrils, Km. 2, Cabrils, Barcelona 08348, Spain

Source 5

Hit accession Automated Authors Title Journal
GQ292148 False Jung,S.
Duwal,R.K.
Lee,S.
COI barcoding of true bugs (Insecta, Heteroptera) Mol Ecol Resour 11 (2), 266-270 (2011)
GQ292148 False Jung,S.H. Direct Submission Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea
GQ292147 False Jung,S.
Duwal,R.K.
Lee,S.
COI barcoding of true bugs (Insecta, Heteroptera) Mol Ecol Resour 11 (2), 266-270 (2011)
GQ292147 False Jung,S.H. Direct Submission Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea
GQ292149 False Jung,S.
Duwal,R.K.
Lee,S.
COI barcoding of true bugs (Insecta, Heteroptera) Mol Ecol Resour 11 (2), 266-270 (2011)
GQ292149 False Jung,S.H. Direct Submission Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea
Show Hide 4 more publications...

Source 6

Hit accession Automated Authors Title Journal
MW357548 False Tabic,A.
Ben-Ami,R.
Direct Submission Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel
MW357547 False Tabic,A.
Ben-Ami,R.
Direct Submission Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel
MW357550 False Tabic,A.
Ben-Ami,R.
Direct Submission Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel
MW357549 False Tabic,A.
Ben-Ami,R.
Direct Submission Submitted (09-DEC-2020) R&D, Bio Bee Sde Eliyahu Ltd., Sde Eliyahu, Sde Eliyahu 1081000, Israel
Show Hide 2 more publications...

Source 7

Hit accession Automated Authors Title Journal
MG452375 False Erfanfar,D.
Srafrazi,A.
Nouri- Ganbalani,G.
Ostovan,H.
Shojaei,M.
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran Unpublished
MG452375 False Erfanfar,D.
Sarafrazi,A.
Direct Submission Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran

Independent sources

Flag 4B: Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources (found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
MG452387 False Erfanfar,D.
Srafrazi,A.
Nouri- Ganbalani,G.
Ostovan,H.
Shojaei,M.
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran Unpublished
MG452387 False Erfanfar,D.
Sarafrazi,A.
Direct Submission Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

Click + drag to pan
Scroll to zoom in/out
Vertical space
Horizontal space

The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net